ITT Cancer Research – Apoptosis – Oncology

Heat stress impairs oocyte maturation through ceramide-mediated apoptosis in pigs

Heat stress impairs oocyte maturation through ceramide-mediated apoptosis in pigs

Heat stress impairs oocyte maturation through ceramide-mediated apoptosis in pigs

Heat stress (HS) is an emerging issue that greatly impairs the reproductive performance of animals and humans. In particular, disruption of oocyte maturation due to HS is considered a major cause of impaired reproductive performance. HS is known to induce ceramide generation, which causes reactive oxygen species (ROS) production and mitochondrial dysfunction, thereby inducing apoptosis. Therefore, we investigated whether inhibition of ceramide generation ameliorates HS-induced apoptosis in porcine cumulus-oocyte complexes (COCs) using specific inhibitors of the de novo (fumonisin B1, FB1) and hydrolytic pathways (desipramine, Des) of ceramide formation. We investigated the effects of FB1 and Des supplementation under HS conditions (41.5 °C for 44 h) on in vitro maturation (IVM) of porcine COCs. After IVM, HS significantly reduced proportion of COCs exhibiting fully expanded cumulus cells and the rate of metaphase II in oocytes. After parthenogenetic activation (PA), HS significantly reduced the rates of cleavage and blastocyst formation with a lower total cell number and a higher percentage of apoptosis in blastocysts.

However, FB1 or Des supplementation under HS avoided detrimental effects of HS on expansion of cumulus cells, nuclear maturation of oocytes, and embryonic development after PA including the rates of cleavage and blastocyst formation, total cell number, and the percentage of apoptosis in blastocysts. Furthermore, FB1 or Des addition under HS, compared with HS alone, significantly decreased ceramide generation, ROS production, cytochrome C expression, and apoptosis and increased mitochondrial membrane potential in COCs, reaching levels comparable with those of the control. Taken together, our results indicate that HS impaired oocyte maturation through ceramide-mediated apoptosis.

Finally, the interaction of SCGN with ATF4 was computationally predicted and then validated by a co-immunoprecipitation assay. We found that ox-LDL treatment increased the levels of ER stress markers, such as phosphorylated protein kinase-like endoplasmic reticulum kinase, phosphorylated eukaryotic initiation factor 2 alpha, activating transcription factor 4 (ATF4), and transcription factor CCAAT-enhancer-binding protein homologous protein, and promoted MIN6 cell apoptosis; in addition, the expression of SCGN was downregulated. siRNA-mediated SCGN knockdown exacerbated β-cell ER stress by increasing ATF4 expression.

Tetrahedral Framework Nucleic Acid Inhibits Chondrocyte Apoptosis and Oxidative Stress through Activation of Autophagy

Osteoarthritis (OA) is a degenerative articular cartilage pathogenic process that is accompanied by excessive chondrocyte apoptosis. The occurrence of chondrocyte death and OA is related to decreased autophagy. Tetrahedral framework nucleic acid (TFNA), a potent bioactive DNA nanomaterial, exerts antiapoptotic and antioxidative effects in various diseases, resulting in autophagy promotion and inhibition of the Wnt/β-catenin-signaling pathway. Here, we aimed to elucidate the therapeutic effects of TFNA on OA and its potential molecular mechanism of action. TFNA was synthesized and characterized by established methods. An interleukin (IL)-1β stimulated OA cell model was established and treated with TFNA. Cellular uptake of TFNA and intracellular reactive oxygen species levels were examined via immunofluorescence and flow cytometry.

Apoptotic cell death was documented by the Cell Counting Kit-8 (CCK8) assay and flow cytometry. Transmission electron microscopy was applied to view the autophagosomes. The expression of BCL2, BAX, caspase-3, Nrf2, HO-1, LC3-II, Beclin1, Atg7, β-catenin, Lef-1, and CyclinD1 was detected by immunofluorescence and western blotting. TFNA was successfully synthesized and effectively entered chondrocytes in the absence or presence of IL-1β without the help of transfection agents. TFNA treatment in IL-1β-induced chondrocytes reduced apoptosis by activating the BCL2/BAX/caspase-3 pathway, inhibited oxidative stress by regulating the Nrf2/HO-1-signaling pathway, and enhanced autophagy through upregulated LC3-II, Beclin1, and Atg7. Moreover, TFNA showed chondroprotective effects by regulating the Wnt/β-catenin-signaling pathway. Overall, TFNA may have utility as a therapeutic nanomedicine for OA.

Heat stress impairs oocyte maturation through ceramide-mediated apoptosis in pigs
Heat stress impairs oocyte maturation through ceramide-mediated apoptosis in pigs

Raddeanin A suppresses lung cancer cell proliferation via induction of apoptosis and increased production of ROS

At present, in vitro cell experiments have confirmed that RaddeaninA can effectively inhibit the proliferation of some tumor cells, but the effect of RaddeaninA on lung cancer cells has not been observed. Therefore, this study explored its effect on lung cancer cells and its mechanism of action. Human lung cancer cell lines were treated with serum-free medium and varied concentrations of Raddeanin A. Cell proliferation and apoptosis were determined using MTT, and flow cytometric assays, respectively. The intracellular level of ROS was determined using DCFH-DA assay. Protein and mRNA expressions of bax, bcl-2 and cyt c were measured using Western blotting and qRT-PCR. RaddeaninA treatment can promote PC-9 cell apoptosis in a time and dose-dependent manner (p<0.05).
Treatment of PC-9 cells with Raddeanin significantly and dose-dependently increased the activities of caspase-9 and caspase-3 (p<0.05), and led to significant and dose-dependent increases in ROS levels. Treatment of PC-9 cells with Raddeanin A led to significant and dose-dependent decreases in mitochondrial membrane potential. It significantly and dose-dependently upregulated bax mRNA and protein expressions, but down-regulated bcl-2 mRNA and protein expressions significantly and dose-dependently (p<0.05). On the other hand, Raddeanin significantly and dose-dependently down-regulated cytoplasmic bax protein expression, while upregulating cyt c expression (p<0.05).

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Goat Anti-Mouse Kappa antibody (AU)

STJ99353-100l 100 µl
EUR 179
Description: AU conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (AU)

STJ99353-1mL 1 mL
EUR 999
Description: AU conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (Biotin)

STJ99403-100l 100 µl
EUR 179
Description: Biotin conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (Biotin)

STJ99403-1mL 1 mL
EUR 999
Description: Biotin conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (FITC)

STJ99453-100l 100 µl
EUR 179
Description: FITC conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (FITC)

STJ99453-1mL 1 mL
EUR 999
Description: FITC conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (HRP)

STJ99503-100l 100 µl
EUR 179
Description: HRP conjugated Goat polyclonal to Mouse Kappa

Goat Anti-Mouse Kappa antibody (HRP)

STJ99503-1mL 1 mL
EUR 999
Description: HRP conjugated Goat polyclonal to Mouse Kappa

Goat anti Human Kappa Chain (FITC)

43C-CB9122 1 mg
EUR 361
Description: Goat anti Human Kappa Chain secondary antibody (FITC)

Goat anti Human Kappa Chain (rhodamine)

43C-CB9123 1 mg
EUR 361
Description: Goat anti Human Kappa Chain secondary antibody (Rhodamine)

Goat anti Human Kappa Chain (biotin)

43C-CB9125 1 mg
EUR 384
Description: Goat anti Human Kappa Chain secondary antibody (biotin)

Goat anti Human Kappa Chain (HRP)

43C-CB9126 1 mg
EUR 396
Description: Goat anti Human Kappa Chain secondary antibody (HRP)

Goat anti Human kappa chain (biotin)

43R-1258 500 ug
EUR 373
Description: Goat anti Human kappa chain secondary antibody (biotin)

Goat anti Human kappa chain (FITC)

43R-1259 500 ug
EUR 350
Description: Goat anti Human kappa chain secondary antibody (FITC)

Goat anti Human kappa chain (HRP)

43R-1260 500 ug
EUR 358
Description: Goat anti Human kappa chain secondary antibody (HRP)

Goat anti Human kappa chain (rhodamine)

43R-1261 500 ug
EUR 350
Description: Goat anti Human kappa chain secondary antibody (rhodamine)

Goat anti Human kappa chain (biotin)

43R-1264 500 ug
EUR 440
Description: Goat anti Human kappa chain secondary antibody (biotin)

Goat anti Human kappa chain (FITC)

43R-1265 500 ug
EUR 402
Description: Goat anti Human kappa chain secondary antibody (FITC)

Goat anti Human kappa chain (HRP)

43R-1266 500 ug
EUR 421
Description: Goat anti Human kappa chain secondary antibody (HRP)

Goat anti Human Kappa light chain

20-S2301G000-S4 10 ml
EUR 138
Description: Goat anti Human Kappa light chain antibody

Goat anti Human Kappa light chain

20-S2301G000-V0 10 ml
EUR 111
Description: Goat anti Human Kappa light chain antibody

Goat anti Human Kappa light chain

20-S2501G000-V0 10 ml
EUR 149
Description: Goat anti Human Kappa light chain antibody

Goat anti Human Kappa light chain

20-S2503GND1-D0 5 ml
EUR 192
Description: Goat anti Human Kappa light chain antibody

Goat Anti-Mouse Kappa-HRP

LF-SA8066H 1.0 ml Ask for price
Description: Goat polyclonal to Mouse Kappa

Goat Anti Human Kappa Light Chain Polyclonal Antibody

CPBT-65574GH 1 mg
EUR 580

Goat Anti Human Kappa Light Chain Polyclonal Antibody

CPBT-65580GH 1 ml
EUR 429

Goat anti Human Kappa Chain (Texas Red)

43C-CB9124 1 mg
EUR 361
Description: Goat anti Human Kappa Chain secondary antibody

Goat anti Human Kappa Chain (Alk Phos)

43C-CB9127 1 mg
EUR 417
Description: Goat anti Human Kappa Chain secondary antibody (Alk Phos)

Goat anti Human kappa chain (Alk Phos)

43R-1257 500 ug
EUR 402
Description: Goat anti Human kappa chain secondary antibody (Alk Phos)

Goat anti Human Kappa Chain (Fab'2)

41C-CJ0129 1 mg
EUR 339
Description: Goat anti Human Kappa Chain secondary antibody (Fab'2)

Goat anti Human Kappa + Lambda light chain

20-S1701G000-V0 10 ml
EUR 138
Description: Goat anti Human kappa + lambda light chain secondary antibody

HRP*Polyclonal Goat Anti-Mouse Kappa

C030214-101ml 10.1ml
EUR 227

HRP*Polyclonal Goat Anti-Mouse Kappa

C030214-1ml 1ml
EUR 945

FITC*Polyclonal Goat Anti-Mouse Kappa

C030614-101ml 10.1ml
EUR 227

FITC*Polyclonal Goat Anti-Mouse Kappa

C030614-1ml 1ml
EUR 945

BIOTIN*Polyclonal Goat Anti-Mouse Kappa

C030814-101ml 10.1ml
EUR 227

BIOTIN*Polyclonal Goat Anti-Mouse Kappa

C030814-1ml 1ml
EUR 945

Goat anti Mouse Kappa Light Chain

70R-KG001 1 ml
EUR 241
Description: Affinity purified Goat anti Mouse Kappa Light Chain antibody

Goat Anti Human Kappa Light Chain Polyclonal Antibody,FITC

CPBT-65572GH 1 mg
EUR 580

Goat Anti Human Kappa Light Chain Polyclonal Antibody,HRP

CPBT-65573GH 1 ml
EUR 580

Kappa; Clone L1C1 (Ready-To-Use)

A00113-0002 2 ml
EUR 69

Kappa; Clone L1C1 (Ready-To-Use)

A00113-0007 7 ml
EUR 95

Kappa; Clone L1C1 (Ready-To-Use)

A00113-0025 25 ml
EUR 188

Anti-Kappa antibody

STJ160049 1 mL C
EUR 235
Description: Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.

Anti-Kappa antibody

STJ180139 0.1 ml
EUR 212

Anti-Kappa antibody

STJ180388 0.1 ml
EUR 212

Goat anti Human Kappa Chain (Fab'2) (FITC)

43C-CJ0130 1 mg
EUR 385
Description: Goat anti Human Kappa Chain secondary antibody (Fab'2) (FITC)

Goat anti Human Kappa Chain (Fab'2) (HRP)

43C-CJ0132 1 mg
EUR 455
Description: Goat anti Human Kappa Chain secondary antibody (Fab'2) (HRP)

Polyclonal Goat Anti-GOT2 (aa 295 to 306) Antibody

AMM04997G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GOT2 (aa 295 to 306) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-GOT2 (aa 360 to 373) Antibody

AMM04998G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GOT2 (aa 360 to 373) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-Renalase (aa 134 to 147) Antibody

AMM05956G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Renalase (aa 134 to 147) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-Renalase (aa 224 to 233) Antibody

APG03367G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Renalase (aa 224 to 233) . This antibody is tested and proven to work in the following applications:

Goat anti Mouse Kappa Light Chain (HRP)

60R-KG001hrp 1 ml
EUR 457
Description: Goat anti Mouse Kappa Light Chain secondary antibody (HRP)

Goat anti Human Kappa Chain (Fab'2) (Texas Red)

43C-CJ0131 1 mg
EUR 389
Description: Goat anti Human Kappa Chain secondary antibody (Fab'2)

anti- immunoglobulin kappa antibody

FNab04295 100µg
EUR 548.75
  • Recommended dilution: WB: 1:500-1:2000
  • IHC: 1:20-1:200
  • Immunogen: immunoglobulin kappa locus
  • Gene ID: 50802
  • Research Area: Immunology
Description: Antibody raised against immunoglobulin kappa

Anti-PP2C kappa Antibody

A04319 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for PP2C kappa Antibody (PPM1K) detection.tested for WB in Human.

Anti-Actin- kappa Antibody

A14672-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Actin- kappa Antibody (ACTBL2) detection. Tested with WB in Human, Mouse.

Anti-immunoglobulin kappa antibody

PAab04295 100 ug
EUR 386

Anti-Actin-kappa antibody

STJ91470 200 µl
EUR 197
Description: Rabbit polyclonal to Actin-kappa.

Anti-DGK-kappa antibody

STJ92704 200 µl
EUR 197
Description: Rabbit polyclonal to DGK-kappa.

Anti-kappa-alpha antibody

STJ93782 200 µl
EUR 197
Description: IkappaB-alpha is a protein encoded by the NFKBIA gene which is approximately 35,6 kDa. IkappaB-alpha is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, 4-1BB pathway and toll-like receptor signalling pathways. This protein falls under the NF-kappa-B inhibitor family. It inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses it becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkappaB-alpha is expressed in adherent monocytes. Mutations in the NFKBIA gene may result in ectodermal dysplasia and leukorrhea. STJ93782 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of IkappaB-alpha protein.

Anti-kappa-alpha antibody

STJ93783 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-alpha.

Anti-kappa-alpha antibody

STJ93784 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-alpha.

Anti-kappa-alpha antibody

STJ93785 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-alpha.

Anti-kappa-beta antibody

STJ93786 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-beta.

Anti-kappa-epsilon antibody

STJ93787 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-epsilon.

Anti-NF kappa antibody

STJ94454 200 µl
EUR 197
Description: Rabbit polyclonal to NFRkappaB.

Anti-PP2 kappa antibody

STJ95197 200 µl
EUR 197
Description: Rabbit polyclonal to PP2Ckappa.

Anti-kappa-alpha antibody

STJ97440 200 µl
EUR 197
Description: Rabbit polyclonal to IkappaB-alpha.

Anti-kappa beta antibody

STJ97539 200 µl
EUR 197
Description: IkappaB beta is a protein encoded by the NFKBIB gene which is approximately 37,7 kDa. IkappaB beta is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, toll-like receptor signalling pathways and the 4-1BB pathway. This protein falls under the NF-kappa-B inhibitor family, which inhibits NF-kappa-B by complexing with it and trapping it in the cytoplasm. Phosphorylation of serine residues on the protein marks it for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which then translocates to the nucleus to function as a transcription factor. IkappaB beta is expressed in the liver, lung, pancreas, nervous system and blood. STJ97539 was developed from clone 1F3 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of IkappaB beta.

Anti-kappa beta antibody

STJ97540 200 µl
EUR 197
Description: Mouse monoclonal to IkappaB beta (8D11).

Anti-kappa-beta antibody

STJ98186 100 µl
EUR 234
Description: Mouse monoclonal to IkappaB-beta.

Anti-kappa-alpha antibody

STJ98512 100 µl
EUR 234
Description: Mouse monoclonal to IkappaB-alpha.

Polyclonal Goat Anti-BDH2 / DHRS6 (aa 60 to 71) Antibody

AMM04910G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-BDH2 / DHRS6 (aa 60 to 71) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody

APG00100G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-TUSC4 / NPRL2 (aa 140 to 151) Antibody

APG00353G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TUSC4 / NPRL2 (aa 140 to 151) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-C2GnT-M (aa 273 to 284) Antibody

APR16228G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-C2GnT-M (aa 273 to 284) . This antibody is tested and proven to work in the following applications:

Polyclonal Goat Anti-C2GnT-M (aa 410 to 422) Antibody

APR16229G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-C2GnT-M (aa 410 to 422) . This antibody is tested and proven to work in the following applications:

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

DLR-GOAT-Hu-48T 48T
EUR 554
  • Should the Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Ghrelin-O-Acyltransferase (GOAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

DLR-GOAT-Hu-96T 96T
EUR 725
  • Should the Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Ghrelin-O-Acyltransferase (GOAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

RD-GOAT-Hu-48Tests 48 Tests
EUR 563

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

RD-GOAT-Hu-96Tests 96 Tests
EUR 783

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

RDR-GOAT-Hu-48Tests 48 Tests
EUR 589

Human Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

RDR-GOAT-Hu-96Tests 96 Tests
EUR 820

OR Antibody

20-abx217389
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

OR Antibody

ABD10250 100 ug
EUR 438

OR Antibody

44609-100ul 100ul
EUR 252

OR Antibody

44609-50ul 50ul
EUR 187

OR Antibody

DF10250 200ul
EUR 304
Description: OR Antibody detects endogenous levels of total OR.

Mouse Anti-Human Ig Kappa antibody

STJ99554-100l 100 µl
EUR 91
Description: Unconjugated Mouse monoclonal to Human Ig Kappa antibody

Mouse Anti-Human Ig Kappa antibody

STJ99554-1mL 1 mL
EUR 514
Description: Unconjugated Mouse monoclonal to Human Ig Kappa antibody

Anti-Human kappa light chain antibody

STJ16100578 1 mL
EUR 290

Goat IgG Secondary antibody Clone: pAb to UltraPolymer Goat Anti-Human IgG (H&L) HRP

A1278-15 15 ml
EUR 322
  • What are the applications and the optimal dilutions of the product Antibody against PARP (Clone 10H)for analysis? - Immunocytochemistry : 5-20 ?g/ml | Western Blot (10 ?g/ml using colorimetric methods | <2 ?g/ml for ECL) | Immunoprecipitation and ELI
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Description: Goat IgG Secondary antibody Clone: pAb against UltraPolymer Goat Anti-Human IgG (H&L) HRPfor application in IHC.The immunogen is Human IgG (H&L).This antibody can be used for detection of the target molecule in samples from Human.

Goat IgG Secondary antibody Clone: pAb to UltraPolymer Goat Anti-Human IgG (H&L) HRP

A1278-5 5 ml
EUR 165
  • What are the applications and the optimal dilutions of the product Antibody against PARP (Clone 10H)for analysis? - Immunocytochemistry : 5-20 ?g/ml | Western Blot (10 ?g/ml using colorimetric methods | <2 ?g/ml for ECL) | Immunoprecipitation and ELI
  • Show more
Description: Goat IgG Secondary antibody Clone: pAb against UltraPolymer Goat Anti-Human IgG (H&L) HRPfor application in IHC.The immunogen is Human IgG (H&L).This antibody can be used for detection of the target molecule in samples from Human.

Kappa, Light Chain; Clone KLC264 (Ready-To-Use)

A00156-0002 2 ml
EUR 103

Kappa, Light Chain; Clone KLC264 (Ready-To-Use)

A00156-0007 7 ml
EUR 181

Kappa, Light Chain; Clone KLC264 (Ready-To-Use)

A00156-0025 25 ml
EUR 444

Nuclear Factor Related To Kappa B Binding Protein (NFRKB) Antibody

20-abx136034
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Nuclear Factor Related To Kappa B Binding Protein (NFRKB) Antibody

20-abx008933
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Nuclear Factor Related To Kappa B Binding Protein (NFRkB) Antibody

20-abx173863
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Nuclear Factor Related To Kappa B Binding Protein (NFRkB) Antibody

20-abx177837
  • EUR 1205.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.

Nuclear Factor Related To Kappa B Binding Protein (NFRKB) Antibody

20-abx326280
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Goat F(Ab')2 Anti Human Kappa Light Chain Polyclonal Antibody,Biotin

CPBT-65575GA 0.5 mg
EUR 580

Goat F(Ab')2 Anti Human Kappa Light Chain Polyclonal Antibody,RPE

CPBT-65578GA 0.5 ml
EUR 580

Polyclonal Goat Anti-TEB4 / MARCH-VI (aa 701 to 712) Antibody

APG00328G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TEB4 / MARCH-VI (aa 701 to 712) . This antibody is tested and proven to work in the following applications:

Anti-Opioid Receptor κ Antibody

A02906 100ug
EUR 406
Description: Rabbit Polyclonal Opioid Receptor κ Antibody. Validated in ELISA, WB and tested in Human, Mouse, Rat.

Anti-PPM1K/Pp2C Kappa Antibody

A04319-1 100ul
EUR 397
Description: Rabbit Polyclonal PPM1K/Pp2C Kappa Antibody. Validated in IHC, WB and tested in Human, Mouse.

Anti-N kappa-p100 antibody

STJ94458 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p100.

Anti-N kappa-p100 antibody

STJ94459 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p100.

Anti-N kappa-p105 antibody

STJ94460 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.

Anti-N kappa-p105 antibody

STJ94461 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.

Anti-N kappa-p105 antibody

STJ94462 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.

Anti-N kappa-p105 antibody

STJ94463 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.

Anti-N kappa-p105 antibody

STJ94464 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.

Anti-N kappa-p65 antibody

STJ94467 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94468 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94469 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94470 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94471 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94472 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94473 200 µl
EUR 197
Description: NFkappaB-p65 is a protein encoded by the RELA gene which is approximately 60,2 kDa. NFkappaB-p65 is localised to the nucleus and cytoplasm. It is involved in activated TLR4 signalling, toll-like receptor signalling pathways, cytosolic sensors of pathogen-associated DNA and immune response. NF-kappa-B is a transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NFkappaB-p65 is expressed in the nervous system, intestine, bone, lung and skin. Mutations in the RELA gene result in Ependymoma, Reticuloendotheliosis and Retinitis Pigmentosa-50. STJ94473 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of NFkappaB-p65 protein.

Anti-N kappa-p65 antibody

STJ94474 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94475 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94476 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ94477 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ96793 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p65.

Anti-N kappa-p65 antibody

STJ98276 100 µl
EUR 234
Description: Mouse monoclonal to NFkappaB-p65.

Anti-NF-kappa p65 antibody

STJ98601 200 µl
EUR 197
Description: Rabbit polyclonal to NF-kappaB p65.

Anti-N kappa-p105 antibody

STJ96474 200 µl
EUR 197
Description: Rabbit polyclonal to NFkappaB-p105.
Similarly, bax protein expression was significantly and dose-dependently upregulated in mitochondria, but the corresponding cyt c expression was significantly and dose-dependently down-regulated (p&lt;0.05). Raddeanin A is a potential and effective lung cancer chemotherapy drug, which can induce lung cancer cell apoptosis and inhibit proliferation.

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