Significance of halofuginone in esophageal squamous carcinoma cell apoptosis through HIF-1α-FOXO3a pathway

Halofuginone (HF) from Dichroa febrifuga has proven therapeutic potential in hepatocellular, lung and colorectal most cancers cell fashions. Proof has additionally indicated that HF performs roles in caustic induced esophageal strictures and oxidative harm. Nonetheless, the position of HF in esophageal squamous carcinoma (ESCC) stays unclear. On this examine, we investigated HF actions and mechanisms throughout ESCC cell apoptosis. We noticed totally different HF concentrations (5, 10 and 20 nM) inhibited ESCC cell survival in a time and dose-dependent method. HF remedy markedly induced KYSE-30 and TE-1 cell apoptosis, and caspase-Three exercise.

Apoptosis associated protein Bax expression was dramatically elevated, whereas Bcl-2 ranges had been diminished in KYSE-30 and TE-1 cells, after HF publicity. Additionally, we confirmed that HF remedy induced DNA injury by selling γH₂AX, pATM and pATR expression. HF remedy additionally diminished hypoxia-inducible factor-1α (HIF-1α) and forkhead field class O 3a (FOXO3a) expression in KYSE-30 and TE-1 cells. We additionally confirmed that HF inhibited FOXO3a expression, however this was depending on HIF-1α inhibition. Lastly, FOXO3a overexpression reversed HF induced cell survival inhibition, cell apoptosis and DNA injury.

FOXO3a knockdown enhanced the consequences of HF on cell survival, cell apoptosis and DNA injury. In abstract, HF performs inhibitory roles in ESCC cell apoptosis, by way of HIF-1α-FOXO3a-dependent signaling. These information assist the notion that HF might act as an efficient therapeutic reagent in the direction of ESCC.

Key phrases: DNA injury; Esophageal squamous carcinoma; FOXO3a; HIF-1α; Halofuginone.

Goals: Uterine leiomyoma (UM) is the most typical benign gynecological tumor and the main indication for hysterectomy. Our examine explored the roles of TRIM9 in leiomyoma formation and investigated the underlying molecular mechanisms.

Materials and strategies: The connection between TRIM9 expression and fibroids formation was deciphered from the GEO database after bioinformatics evaluation and recognized by qPCR in human leiomyoma tissues. Each TRIM9 mRNA and protein expression had been additional detected in main cultured uterine leiomyoma cells (UMC). The tumorigenesis potentials of TRIM9 in cell proliferation, cell cycle, cell apoptosis; cyclin D1, survivin and cleaved-caspase Three protein expressions in main UMC with TRIM9 overexpression (UMC-oeTRIM9); and uterine clean muscle cells (SMC) with TRIM9 knockdown (SMC-siTRIM9) had been evaluated in vitro. NF-kB p65 and its phosphorylation had been additional examined by western blotting, and rescue experiments on cell proliferation, cell cycle and cell apoptosis had been performed.

  Product not found

Key findings: TRIM9 confirmed greater expression in UM tissue and UMC in contrast with regular myometrium. The overexpression of TRIM9 in UMC notably promoted UM progress by way of enhancement of cell proliferation, discount of cell apoptosis, and regulation of cyclin D1, survivin, cleaved-caspase 3, and nuclear NF-κB expression, which had been reversed in SMC-siTRIM9 and PDTC (an NF-κB inhibitor) intervention in UMC-oeTRIM9.

Significance: To our information, this was the primary examine demonstrating the roles of TRIM9 in cell progress development of UM improvement. TRIM9 could also be a possible therapeutic goal for UM, by selling leiomyoma cell proliferation and decreasing cell apoptosis by way of activation of the NF-κB pathway.

 

Mouse Caspase 7 (CASP7) ELISA Kit
DLR-CASP7-Mu-48T	DL Develop	48T	EUR 450
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Caspase 7 (CASP7) in samples from tissue homogenates or other biological fluids.

Mouse Caspase 7 (CASP7) ELISA Kit
DLR-CASP7-Mu-96T	DL Develop	96T	EUR 582
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Caspase 7 (CASP7) in samples from tissue homogenates or other biological fluids.

Rat Caspase 7 (CASP7) ELISA Kit
DLR-CASP7-Ra-48T	DL Develop	48T	EUR 467
Description: A sandwich quantitative ELISA assay kit for detection of Rat Caspase 7 (CASP7) in samples from tissue homogenates or other biological fluids.

Rat Caspase 7 (CASP7) ELISA Kit
DLR-CASP7-Ra-96T	DL Develop	96T	EUR 605
Description: A sandwich quantitative ELISA assay kit for detection of Rat Caspase 7 (CASP7) in samples from tissue homogenates or other biological fluids.

Human Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Hu-48Tests	Reddot Biotech	48 Tests	EUR 455

Human Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Hu-96Tests	Reddot Biotech	96 Tests	EUR 629

Mouse Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Mu-48Tests	Reddot Biotech	48 Tests	EUR 465

Mouse Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Mu-96Tests	Reddot Biotech	96 Tests	EUR 643

Rat Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Ra-48Tests	Reddot Biotech	48 Tests	EUR 486

Rat Caspase 7 (CASP7) ELISA Kit
RDR-CASP7-Ra-96Tests	Reddot Biotech	96 Tests	EUR 672

Human Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Hu-48Tests	Reddot Biotech	48 Tests	EUR 436

Human Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Hu-96Tests	Reddot Biotech	96 Tests	EUR 601

Mouse Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Mu-48Tests	Reddot Biotech	48 Tests	EUR 446

Mouse Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Mu-96Tests	Reddot Biotech	96 Tests	EUR 615

Rat Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Ra-48Tests	Reddot Biotech	48 Tests	EUR 465

Rat Caspase 7 (CASP7) ELISA Kit
RD-CASP7-Ra-96Tests	Reddot Biotech	96 Tests	EUR 643

Anti-Caspase-7/CASP7 Antibody
PB9369	BosterBio	100ug/vial	EUR 334

Caspase 7 (CASP7) Antibody
20-abx009498	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Caspase 7 (CASP7) Antibody
20-abx009500	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Caspase 7 (CASP7) Antibody
20-abx211748	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Caspase 7 (CASP7) Antibody
20-abx212611	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Caspase 7 (CASP7) Antibody
20-abx100463	Abbexa	EUR 356.00 EUR 133.00 EUR 996.00 EUR 495.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx100464	Abbexa	EUR 356.00 EUR 133.00 EUR 996.00 EUR 495.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx100465	Abbexa	EUR 356.00 EUR 133.00 EUR 1024.00 EUR 509.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (Casp7) Antibody
abx117104-100ug	Abbexa	100 ug	EUR 467

Caspase 7 (CASP7) Antibody
abx037415-100ug	Abbexa	100 ug	EUR 391

Caspase 7 (CASP7) Antibody
abx037416-100ug	Abbexa	100 ug	EUR 391

Caspase 7 (CASP7) Antibody
abx038175-100ug	Abbexa	100 ug	EUR 391

Caspase 7 (CASP7) Antibody
abx038389-100ug	Abbexa	100 ug	EUR 391

Caspase 7 (CASP7) Antibody
20-abx129521	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx130903	Abbexa	EUR 398.00 EUR 133.00 EUR 1066.00 EUR 523.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx109607	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx171611	Abbexa	EUR 718.00 EUR 384.00	1 mg 200 ug

Caspase 7 (CASP7) Antibody
20-abx171612	Abbexa	EUR 843.00 EUR 439.00	1 mg 200 ug

Caspase 7 (CASP7) Antibody
abx340179-100ug	Abbexa	100 ug	EUR 391

Caspase 7 (CASP7) Antibody
20-abx325945	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx326016	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

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20-abx323899	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Caspase 7 (CASP7) Antibody
20-abx324958	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

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20-abx325578	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

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20-abx001283	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Anti-Caspase-7(P11)/CASP7 Antibody
PA1442	BosterBio	100ug/vial	EUR 334

Caspase 7 (CASP7) Polyclonal Antibody (Human)
4-PAA449Hu01	Cloud-Clone	EUR 217.00 EUR 2048.00 EUR 520.00 EUR 268.00 EUR 201.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7)

Caspase 7 (CASP7) Polyclonal Antibody (Human)
4-PAA449Hu02	Cloud-Clone	EUR 217.00 EUR 2048.00 EUR 520.00 EUR 268.00 EUR 201.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7)

Caspase 7 (CASP7) Polyclonal Antibody (Mouse)
4-PAA449Mu01	Cloud-Clone	EUR 221.00 EUR 2100.00 EUR 532.00 EUR 272.00 EUR 202.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Caspase 7 (CASP7)

Caspase 7 (CASP7) Polyclonal Antibody (Mouse)
4-PAA449Mu02	Cloud-Clone	EUR 236.00 EUR 2338.00 EUR 586.00 EUR 294.00 EUR 209.00	100ul 10ml 1ml 200ul 20ul
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Caspase 7 (CASP7) Polyclonal Antibody (Rat)
4-PAA449Ra01	Cloud-Clone	EUR 227.00 EUR 2206.00 EUR 556.00 EUR 282.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Caspase 7 (CASP7)

Rabbit Caspase 7 (CASP7) ELISA Kit
abx363195-96tests	Abbexa	96 tests	EUR 825

Human Caspase-7 (CASP7)
1-CSB-RP154794h(A3)	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Caspase-7(CASP7),partial expressed in E.coli

Human Caspase-7 (CASP7)
1-CSB-RP154794h(A4)	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Caspase-7(CASP7),partial expressed in E.coli

Recombinant Caspase 7 (CASP7)
4-RPA449Hu01	Cloud-Clone	EUR 548.00 EUR 250.00 EUR 1780.00 EUR 660.00 EUR 1220.00 EUR 430.00 EUR 4300.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Caspase 7 expressed in: E.coli

Recombinant Caspase 7 (CASP7)
4-RPA449Hu02	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Caspase 7 expressed in: E.coli

Recombinant Caspase 7 (CASP7)
4-RPA449Mu01	Cloud-Clone	EUR 565.92 EUR 254.00 EUR 1847.20 EUR 682.40 EUR 1264.80 EUR 442.00 EUR 4468.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Caspase 7 expressed in: E.coli

Recombinant Caspase 7 (CASP7)
4-RPA449Mu02	Cloud-Clone	EUR 565.92 EUR 254.00 EUR 1847.20 EUR 682.40 EUR 1264.80 EUR 442.00 EUR 4468.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Caspase 7 expressed in: E.coli

Recombinant Caspase 7 (CASP7)
4-RPA449Ra01	Cloud-Clone	EUR 574.88 EUR 257.00 EUR 1880.80 EUR 693.60 EUR 1287.20 EUR 448.00 EUR 4552.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Caspase 7 expressed in: E.coli

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20-abx271987	Abbexa	EUR 398.00 EUR 230.00 EUR 1080.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

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20-abx272697	Abbexa	EUR 398.00 EUR 230.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

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20-abx370253	Abbexa	EUR 1428.00 EUR 926.00	10 × 96 tests 5 × 96 tests

Caspase 7 (CASP7) Antibody (FITC)
20-abx273801	Abbexa	EUR 411.00 EUR 230.00 EUR 1163.00 EUR 565.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

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20-abx274006	Abbexa	EUR 425.00 EUR 230.00 EUR 1191.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Caspase 7 (CASP7) Polyclonal Antibody (Human), APC
4-PAA449Hu01-APC	Cloud-Clone	EUR 301.00 EUR 2645.00 EUR 755.00 EUR 377.00 EUR 200.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with APC.

Caspase 7 (CASP7) Polyclonal Antibody (Human), Biotinylated
4-PAA449Hu01-Biotin	Cloud-Clone	EUR 279.00 EUR 1998.00 EUR 612.00 EUR 334.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with Biotin.

Caspase 7 (CASP7) Polyclonal Antibody (Human), Cy3
4-PAA449Hu01-Cy3	Cloud-Clone	EUR 360.00 EUR 3485.00 EUR 965.00 EUR 461.00 EUR 225.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with Cy3.

Caspase 7 (CASP7) Polyclonal Antibody (Human), FITC
4-PAA449Hu01-FITC	Cloud-Clone	EUR 261.00 EUR 2136.00 EUR 624.00 EUR 321.00 EUR 180.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with FITC.

Caspase 7 (CASP7) Polyclonal Antibody (Human), HRP
4-PAA449Hu01-HRP	Cloud-Clone	EUR 277.00 EUR 2309.00 EUR 671.00 EUR 343.00 EUR 190.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with HRP.

Caspase 7 (CASP7) Polyclonal Antibody (Human), PE
4-PAA449Hu01-PE	Cloud-Clone	EUR 261.00 EUR 2136.00 EUR 624.00 EUR 321.00 EUR 180.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with PE.

Caspase 7 (CASP7) Polyclonal Antibody (Human), APC
4-PAA449Hu02-APC	Cloud-Clone	EUR 301.00 EUR 2645.00 EUR 755.00 EUR 377.00 EUR 200.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Caspase 7 (CASP7). This antibody is labeled with APC.

Key phrases: Cell apoptosis; Cell proliferation; NF-κB signaling pathway; TRIM9; Uterine leiomyoma.